产品货号：
GS2144
中文名称：
AccB7I内切酶
英文名称：
AccB7I
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity.
技术规格
相关搜索：AccB7I内切酶，AccB7I限制性内切酶
使用说明
High enzyme concentration may result in star activity.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dcm-)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | CCANNNN↑NTGG |
| 来源 | Acinetobacter calcoaceticus B7 |
| Assayed on | Lambda DNA(dcm-) |
| Optimal buffer | G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlapping Dcm methylation(CmCWGG):CCANNNCCTGG or CCAGGNNNTGG. |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BasI,PflBI,PflMI,Van91I |
相关搜索：AccB7I内切酶，AccB7I限制性内切酶